C. difficile is a spore-forming obligate anaerobic bacterium, which can be part of the normal intestinal flora in as many as 50% of children under age two, about 5% of health adults and about 10% or more of hospitalized adults. C. difficile-associated diarrhea and colitis (CDDC) occur when normal intestinal flora are altered by frequent and prolong use of antibiotics including penicillin, clindamycin, and cephalosporins, allowing C. difficile (CD) to overgrow and produce toxins, resulting in the development of CDDC.Unlike the self-limiting diarrhea caused by other kinds of bacteria, CDDC are usually serious and potentially life threatening. Current treatment for CDDC includes the use of antibiotic regimens that are not known to result in CDDC. The increased reports on the emergence of drug-resistant C. difficile post new additional challenge for clinicians to treat the CDDC. A new and a non-antibiotic treatment approach such as a biotherapy using other bacteria should be evaluated as an alternative. This proposed project will provide more opportunities to Cal Poly students to participate in many of the sophisticated biological and molecular research techniques, complementing and strengthening our recently approved Biotechnology majors. Our students will become more competitive after their graduations in the increasing competitive market place or in their future pursuit to further their educations in professional schools, especially in the biomedical fields.